Visualization and quantification of cardiac mitochondrial protein clusters with STED microscopy
H Singh, R Lu, PFG Rodríguez, Y Wu, JC Bopassa… - Mitochondrion, 2012 - Elsevier
The visualization and quantification of mitochondria-associated proteins with high power
microscopy methods is of particular interest to investigate protein architecture in this
organelle. We report the usage of a custom-made STimulated Emission Depletion (STED)
fluorescence nanoscope with~ 30nm lateral resolution for protein mapping of Percoll-
purified viable mitochondria from murine heart. Using this approach, we were able to
quantify and resolve distinct protein clusters within mitochondria; specifically, cytochrome c …
microscopy methods is of particular interest to investigate protein architecture in this
organelle. We report the usage of a custom-made STimulated Emission Depletion (STED)
fluorescence nanoscope with~ 30nm lateral resolution for protein mapping of Percoll-
purified viable mitochondria from murine heart. Using this approach, we were able to
quantify and resolve distinct protein clusters within mitochondria; specifically, cytochrome c …
